Journal: Journal of Functional Foods

Article Title: Camellia oil improves Aβ25-35-induced memory impairment by regulating the composition of the gut microbiota and lipid metabolism in mice

doi: 10.1016/j.jff.2022.105214

Figure Lengend Snippet: Fig. 4. Camellia oil mediated lipid metabolism through S1P and PPARs receptors to improve Aβ25-35-induced cognitive impairment in mice. (A-B). The proteins of SPHK1, S1PR5, PPAR-α, and PPAR-γ were detected by Western blot in brain tissue, and the relative expression of SPHK1, S1PR5, PPAR-α, and PPAR-γ was sta tistically analyzed. (C). The levels of S1P in the brain tissue, n = 5. (D). The levels of GSH-Px, MDA, and SOD in serum, n = 6. (E). The levels of ROS in primary mouse brain cells, and quantitative analysis of ROS. n = 3. The data were expressed as the mean ± SD. *P < 0.05, **P < 0.01 compared with the M group. Con means the control, M means the model, Don means the donepezil, Low means low-dose camellia oil-treated group, High means high-dose camellia oil-treated group.

Article Snippet: Protein samples from each group were separated by 10 % SDS-PAGE, transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore Ltd, Germany), and incubated with primary antibodies against SPHK1 (1:1000,10670-1-AP, Proteinate), S1PR5 (1:1000, CSB-PA867132LA01HU, CUSABIO), PPARγ (1:1000, A0270, Abclonal), PPAR-α (1:1000, #AF5301, Affinity Bioscience), β-actin (1:25000, AC026, Abclonal, Wuhan, China), and GAPDH (1:25000, AC033, Abclonal, Wuhan, China) overnight at 4 ◦C.

Techniques: Western Blot, Expressing, Control